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KMID : 1134819980270010175
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Journal of the Korean Society of Food Science and Nutrition
1998 Volume.27 No. 1 p.175 ~ p.181
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Activities of Phospholipase A₂ and Cyclooxygenase, and Syntheses of Thromboxane and Prostacyclin in Streptozotocin Induced Diabetic Rats
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Yang Jeong-Ah
Kim Sung-Ok
Choi Jeong-Hwa
Kwag Oh-Gye
Rhee Soon-Jae
Chang Hyun-Wook
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Abstract
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The relation between lipid peroxidation and thrombotic reaction were investigated in streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing l00±10gm were randomly assigned to normal and STZ-induced diabetic group(DM). Diabetes was experimentally induced by intravenous injection of 55㎎/㎏ of body weight of STZ in citrate buffer(pH 4.3) after 4 weeks feeding of basal diet. Animals were sacrificed at the 6th day of diabetic states. Body weight gains were lower in diabetic group after STZ injection. Serum levels of thiobarbituric acid reacting substances(TBARS) that were markedly increased in DM group compared with of normal group. TBARS levles of HDL and LDL were similar patterns to total TBARS of serum. Activities of platelet phospholipase A₂(PLA₂) were higher in diabetic group than those of normal group. Activities of platelet cyclooxygenase were 106% in DM group than normal group. Platelet thromboxane A₂(TXA₂) formation was increased in DM group than normal group. Production of aortic prostacyclin(PGI₂) was lower in diabetic group than that of normal group. PGI₂/TXA₂ ratios were decreased by 55% in DM groups than those of normal group. The present results indicate that STZ-induced diabetic rats are more sensitive to oxidative stess which leads to acceleration of lipid peroxidation and platelet aggregability. In conclusion, accelerating effect of lipid peroxidation and thrombogenesis in diabetic state is regarded to be resulted from enhancement of PLA₂ activity and arachidonic acid metabolism, inhibition of antiaggregating agent and aortic PGI₂ formatiom.
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KEYWORD
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diabetes mellitus, lipid peroxidation, phospholipase A2, cyclooxygenase, prostaglandins
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